lif rabbit polyclonal antibodies Search Results


rabbit anti lif  (OriGene)
92
OriGene rabbit anti lif
Pre-pubertal Lgr5 high uterus cells behave as Wnt-regulated stem/progenitor cells in ex vivo organoid culture assays. a Representative image of a developing uterine organoid generated from a single EPCAM + GFP high cell isolated from a P14 Lgr5-2A-EGFP uterus. b H&E and IHC for K8 on a Lgr5 high cell-derived organoid. c Endogenous EGFP fluorescence on a Lgr5 high cell-derived organoid. Yellow arrowheads highlight the heterogenous expression of Lgr5-EGFP within individual organoids. Scale bars, 50 μm. d Organoids generated from single EPCAM + GFP high cells and EPCAM + GFP neg cells (image representative of five replicates). Scale bars, 1000 μm. e Quantification of organoid formation efficacy from single EPCAM + GFP high and EPCAM + GFP neg cells. Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test. *** P < 0.001. f Passage frequency of organoids derived from single EPCAM + GFP high and EPCAM + GFP neg cells. Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test ( P = 7E−07). g Experimental strategy to ablate Lgr5 + cells in vitro. h Organoid cultures derived from Lgr5-DTR-EGFP and wild-type (control) EPCAM + uterus cells following Lgr5 + cell ablation by DT treatment in vitro (image representative of five replicates). Scale bars, 200 μm. i Outgrowth efficiency of organoids derived from Lgr5-DTR-EGFP and wild-type mouse EPCAM + uterus cells following DT treatment in vitro. Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test ( P = 4E−06). j Organoids derived from wild-type EPCAM + uterus cells cultured under low Wnt3a and low Wnt3a + CHIR conditions (image representative of five replicates). Scale bars, 200 μm. k The average proportions of organoid types under low Wnt3a, low Wnt3a + CHIR and high Wnt3a conditions. Data from five independent experiments are presented. Data were tested for significance by a chi-square test (Low Wnt3a vs. Low Wnt3a + CHIR: P = 2E−17, Low Wnt3a vs. High Wnt3a: P = 2E−08). l QPCR analysis of Lgr5 and Fzd10 expression on organoids generated under each culture condition. Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test ( Lgr5 : Low Wnt3a vs. Low Wnt3a + CHIR: P = 0.002, Low Wnt3a vs. High Wnt3a: P = 0.012, Fzd10 : Low Wnt3a vs. Low Wnt3a + CHIR: P = 0.018, Low Wnt3a vs. High Wnt3a: P = 0.027). m QPCR analysis of GE markers ( Foxa2, <t>Lif,</t> Prss28 ) and LE markers ( Wnt7a, Scnn1a, Irg1 ) expression on organoids generated under each culture condition Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test ( Foxa2 : P = 9E−04, Lif : P = 0.006, Prss28 : P = 0.54, Wnt7a : P = 0.46, Scnn1a : P = 0.49, Irg1 : P = 5E−06). n , o Immunostaining for Foxa2, Lif, <t>and</t> <t>Ki67</t> on round and vacuolated-type organoids. Scale bars, 50 μm. Red arrows indicate Foxa2-positive cells (image representative of three independent mice). *** P < 0.001, ** P < 0.01, * P < 0.05, N.S., not significant.
Rabbit Anti Lif, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti lif/product/OriGene
Average 92 stars, based on 1 article reviews
rabbit anti lif - by Bioz Stars, 2026-03
92/100 stars
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rabbit anti-human lif polyclonal antibody p15018  (Abbiotec Inc)
90
Abbiotec Inc rabbit anti-human lif polyclonal antibody p15018
Pre-pubertal Lgr5 high uterus cells behave as Wnt-regulated stem/progenitor cells in ex vivo organoid culture assays. a Representative image of a developing uterine organoid generated from a single EPCAM + GFP high cell isolated from a P14 Lgr5-2A-EGFP uterus. b H&E and IHC for K8 on a Lgr5 high cell-derived organoid. c Endogenous EGFP fluorescence on a Lgr5 high cell-derived organoid. Yellow arrowheads highlight the heterogenous expression of Lgr5-EGFP within individual organoids. Scale bars, 50 μm. d Organoids generated from single EPCAM + GFP high cells and EPCAM + GFP neg cells (image representative of five replicates). Scale bars, 1000 μm. e Quantification of organoid formation efficacy from single EPCAM + GFP high and EPCAM + GFP neg cells. Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test. *** P < 0.001. f Passage frequency of organoids derived from single EPCAM + GFP high and EPCAM + GFP neg cells. Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test ( P = 7E−07). g Experimental strategy to ablate Lgr5 + cells in vitro. h Organoid cultures derived from Lgr5-DTR-EGFP and wild-type (control) EPCAM + uterus cells following Lgr5 + cell ablation by DT treatment in vitro (image representative of five replicates). Scale bars, 200 μm. i Outgrowth efficiency of organoids derived from Lgr5-DTR-EGFP and wild-type mouse EPCAM + uterus cells following DT treatment in vitro. Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test ( P = 4E−06). j Organoids derived from wild-type EPCAM + uterus cells cultured under low Wnt3a and low Wnt3a + CHIR conditions (image representative of five replicates). Scale bars, 200 μm. k The average proportions of organoid types under low Wnt3a, low Wnt3a + CHIR and high Wnt3a conditions. Data from five independent experiments are presented. Data were tested for significance by a chi-square test (Low Wnt3a vs. Low Wnt3a + CHIR: P = 2E−17, Low Wnt3a vs. High Wnt3a: P = 2E−08). l QPCR analysis of Lgr5 and Fzd10 expression on organoids generated under each culture condition. Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test ( Lgr5 : Low Wnt3a vs. Low Wnt3a + CHIR: P = 0.002, Low Wnt3a vs. High Wnt3a: P = 0.012, Fzd10 : Low Wnt3a vs. Low Wnt3a + CHIR: P = 0.018, Low Wnt3a vs. High Wnt3a: P = 0.027). m QPCR analysis of GE markers ( Foxa2, <t>Lif,</t> Prss28 ) and LE markers ( Wnt7a, Scnn1a, Irg1 ) expression on organoids generated under each culture condition Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test ( Foxa2 : P = 9E−04, Lif : P = 0.006, Prss28 : P = 0.54, Wnt7a : P = 0.46, Scnn1a : P = 0.49, Irg1 : P = 5E−06). n , o Immunostaining for Foxa2, Lif, <t>and</t> <t>Ki67</t> on round and vacuolated-type organoids. Scale bars, 50 μm. Red arrows indicate Foxa2-positive cells (image representative of three independent mice). *** P < 0.001, ** P < 0.01, * P < 0.05, N.S., not significant.
Rabbit Anti Human Lif Polyclonal Antibody P15018, supplied by Abbiotec Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-human lif polyclonal antibody p15018/product/Abbiotec Inc
Average 90 stars, based on 1 article reviews
rabbit anti-human lif polyclonal antibody p15018 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Pre-pubertal Lgr5 high uterus cells behave as Wnt-regulated stem/progenitor cells in ex vivo organoid culture assays. a Representative image of a developing uterine organoid generated from a single EPCAM + GFP high cell isolated from a P14 Lgr5-2A-EGFP uterus. b H&E and IHC for K8 on a Lgr5 high cell-derived organoid. c Endogenous EGFP fluorescence on a Lgr5 high cell-derived organoid. Yellow arrowheads highlight the heterogenous expression of Lgr5-EGFP within individual organoids. Scale bars, 50 μm. d Organoids generated from single EPCAM + GFP high cells and EPCAM + GFP neg cells (image representative of five replicates). Scale bars, 1000 μm. e Quantification of organoid formation efficacy from single EPCAM + GFP high and EPCAM + GFP neg cells. Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test. *** P < 0.001. f Passage frequency of organoids derived from single EPCAM + GFP high and EPCAM + GFP neg cells. Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test ( P = 7E−07). g Experimental strategy to ablate Lgr5 + cells in vitro. h Organoid cultures derived from Lgr5-DTR-EGFP and wild-type (control) EPCAM + uterus cells following Lgr5 + cell ablation by DT treatment in vitro (image representative of five replicates). Scale bars, 200 μm. i Outgrowth efficiency of organoids derived from Lgr5-DTR-EGFP and wild-type mouse EPCAM + uterus cells following DT treatment in vitro. Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test ( P = 4E−06). j Organoids derived from wild-type EPCAM + uterus cells cultured under low Wnt3a and low Wnt3a + CHIR conditions (image representative of five replicates). Scale bars, 200 μm. k The average proportions of organoid types under low Wnt3a, low Wnt3a + CHIR and high Wnt3a conditions. Data from five independent experiments are presented. Data were tested for significance by a chi-square test (Low Wnt3a vs. Low Wnt3a + CHIR: P = 2E−17, Low Wnt3a vs. High Wnt3a: P = 2E−08). l QPCR analysis of Lgr5 and Fzd10 expression on organoids generated under each culture condition. Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test ( Lgr5 : Low Wnt3a vs. Low Wnt3a + CHIR: P = 0.002, Low Wnt3a vs. High Wnt3a: P = 0.012, Fzd10 : Low Wnt3a vs. Low Wnt3a + CHIR: P = 0.018, Low Wnt3a vs. High Wnt3a: P = 0.027). m QPCR analysis of GE markers ( Foxa2, Lif, Prss28 ) and LE markers ( Wnt7a, Scnn1a, Irg1 ) expression on organoids generated under each culture condition Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test ( Foxa2 : P = 9E−04, Lif : P = 0.006, Prss28 : P = 0.54, Wnt7a : P = 0.46, Scnn1a : P = 0.49, Irg1 : P = 5E−06). n , o Immunostaining for Foxa2, Lif, and Ki67 on round and vacuolated-type organoids. Scale bars, 50 μm. Red arrows indicate Foxa2-positive cells (image representative of three independent mice). *** P < 0.001, ** P < 0.01, * P < 0.05, N.S., not significant.

Journal: Nature Communications

Article Title: Neonatal Wnt-dependent Lgr5 positive stem cells are essential for uterine gland development

doi: 10.1038/s41467-019-13363-3

Figure Lengend Snippet: Pre-pubertal Lgr5 high uterus cells behave as Wnt-regulated stem/progenitor cells in ex vivo organoid culture assays. a Representative image of a developing uterine organoid generated from a single EPCAM + GFP high cell isolated from a P14 Lgr5-2A-EGFP uterus. b H&E and IHC for K8 on a Lgr5 high cell-derived organoid. c Endogenous EGFP fluorescence on a Lgr5 high cell-derived organoid. Yellow arrowheads highlight the heterogenous expression of Lgr5-EGFP within individual organoids. Scale bars, 50 μm. d Organoids generated from single EPCAM + GFP high cells and EPCAM + GFP neg cells (image representative of five replicates). Scale bars, 1000 μm. e Quantification of organoid formation efficacy from single EPCAM + GFP high and EPCAM + GFP neg cells. Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test. *** P < 0.001. f Passage frequency of organoids derived from single EPCAM + GFP high and EPCAM + GFP neg cells. Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test ( P = 7E−07). g Experimental strategy to ablate Lgr5 + cells in vitro. h Organoid cultures derived from Lgr5-DTR-EGFP and wild-type (control) EPCAM + uterus cells following Lgr5 + cell ablation by DT treatment in vitro (image representative of five replicates). Scale bars, 200 μm. i Outgrowth efficiency of organoids derived from Lgr5-DTR-EGFP and wild-type mouse EPCAM + uterus cells following DT treatment in vitro. Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test ( P = 4E−06). j Organoids derived from wild-type EPCAM + uterus cells cultured under low Wnt3a and low Wnt3a + CHIR conditions (image representative of five replicates). Scale bars, 200 μm. k The average proportions of organoid types under low Wnt3a, low Wnt3a + CHIR and high Wnt3a conditions. Data from five independent experiments are presented. Data were tested for significance by a chi-square test (Low Wnt3a vs. Low Wnt3a + CHIR: P = 2E−17, Low Wnt3a vs. High Wnt3a: P = 2E−08). l QPCR analysis of Lgr5 and Fzd10 expression on organoids generated under each culture condition. Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test ( Lgr5 : Low Wnt3a vs. Low Wnt3a + CHIR: P = 0.002, Low Wnt3a vs. High Wnt3a: P = 0.012, Fzd10 : Low Wnt3a vs. Low Wnt3a + CHIR: P = 0.018, Low Wnt3a vs. High Wnt3a: P = 0.027). m QPCR analysis of GE markers ( Foxa2, Lif, Prss28 ) and LE markers ( Wnt7a, Scnn1a, Irg1 ) expression on organoids generated under each culture condition Data from five independent experiments are presented as mean ± s.e.m. Data were tested for significance using unpaired two-tailed t -test ( Foxa2 : P = 9E−04, Lif : P = 0.006, Prss28 : P = 0.54, Wnt7a : P = 0.46, Scnn1a : P = 0.49, Irg1 : P = 5E−06). n , o Immunostaining for Foxa2, Lif, and Ki67 on round and vacuolated-type organoids. Scale bars, 50 μm. Red arrows indicate Foxa2-positive cells (image representative of three independent mice). *** P < 0.001, ** P < 0.01, * P < 0.05, N.S., not significant.

Article Snippet: The following primary antibodies were employed: mouse anti-Lim1 (1:100; DSHB, 4F2), rabbit anti-Foxa2 (1:400; Cell Signaling, 8186), rabbit anti-K8 (1:400; Abcam, ab53280), rabbit anti-vimentin (1:1000; Abcam, ab92547), mouse anti-E-cadherin (1:200; BD Transduction Laboratories, 610181), rabbit anti-cleaved Caspase3 (1:200; Cell Signaling, 9661), rabbit anti-Ki67 (1:200; ThermoFisher, MA5-14520), rabbit anti-LIF (1:200; Origene, TA321468), mouse anti-Ki67 (1:200; BD Transduction Laboratories, 550609), chicken anti-GFP (1:100; Abcam, ab13970), rabbit anti-GFP (1:200; Cell Signaling, 2956S), rabbit anti-RFP (1:200; Rockland, 600-401-379), mouse anti-RFP (1:100; Abcam, ab125244).

Techniques: Ex Vivo, Generated, Isolation, Derivative Assay, Fluorescence, Expressing, Two Tailed Test, In Vitro, Cell Culture, Immunostaining